Hotspot

rbcL-psaI

LSC · 58056-59346 · 1291 bp

Back to Otatea

Pi 0.0031
MarkerSeek score 60.60
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
1291
variable_sites
4
pi
0.0031
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 CGCAGCTTGTGAAGTATGGA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.3 58.0 1428-1434 bp 1.0 77.9
rbcL-psaI_p2 CGCAGCTTGTGAAGTATGGA AAGAAGCCATTGCAATTGCC 50.0 45.0 58.3 57.9 1434-1440 bp 1.0 77.3
rbcL-psaI_p3 GGCGATCAAATTCGAGTTCG CCATTGCAATTGCCGGAAAT 50.0 45.0 57.6 58.0 1407-1413 bp 1.0 77.2
rbcL-psaI_p4 TGGAAGGCGATCAAATTCGA CCATTGCAATTGCCGGAAAT 45.0 45.0 57.6 58.0 1412-1418 bp 1.0 76.9
rbcL-psaI_p5 GGCGATCAAATTCGAGTTCG AAGAAGCCATTGCAATTGCC 50.0 45.0 57.6 57.9 1413-1419 bp 1.0 76.6