Hotspot

ndhC-trnV-UAC

LSC · 52016-52900 · 885 bp

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Pi 0.00904
MarkerSeek score 63.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
885
variable_sites
8
pi
0.00904
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAAGTCC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.0 57.9 1011-1021 bp 1.0 79.7
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAAGTCC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.0 58.3 1012-1022 bp 1.0 77.9
ndhC-trnV-UAC_p3 TAACCGGGGCTAAAAGTCCT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 1010-1020 bp 1.0 77.3
ndhC-trnV-UAC_p4 CACTAACCGGGGCTAAAAGT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 1013-1023 bp 1.0 76.1
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAAGTCCT CGGTTCGAATCCGTATAGCC 50.0 55.0 58.3 58.3 1011-1021 bp 1.0 75.5