Hotspot

trnL-UAA-trnF-GAA

LSC · 49323-49677 · 355 bp

Back to Otatea

Pi 0.00565
MarkerSeek score 57.20
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
355
variable_sites
2
pi
0.00565
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AAGTCGTGAGGGTTCAAGTC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 445-446 bp 1.0 75.9
trnL-UAA-trnF-GAA_p2 AAGTCGTGAGGGTTCAAGTC CTGTGCCAGGAACCAGATTT 50.0 50.0 57.5 58.1 462-463 bp 1.0 75.6
trnL-UAA-trnF-GAA_p3 AAGTCGTGAGGGTTCAAGTC GTGCCAGGAACCAGATTTGA 50.0 50.0 57.5 58.1 460-461 bp 1.0 75.6
trnL-UAA-trnF-GAA_p4 AAGTCGTGAGGGTTCAAGTC TTTTTCTGTGCCAGGAACCA 50.0 45.0 57.5 57.8 467-468 bp 1.0 75.6
trnL-UAA-trnF-GAA_p5 AAGTCGTGAGGGTTCAAGTC TTTTCTGTGCCAGGAACCAG 50.0 50.0 57.5 58.3 466-467 bp 1.0 74.3