Hotspot

atpF-atpA

LSC · 36266-36357 · 92 bp

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Pi 0.0217
MarkerSeek score 65.00
Primer available yes
Label atpF-atpA

Feature Metadata

label_name
atpF-atpA
feature_type
igs
region
LSC
length_bp
92
variable_sites
2
pi
0.0217
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpA_p1 GTGCAAGGAGCTCTAGGAAC CATCCCCCACTTGAACTACG 55.0 55.0 58.0 58.0 312 bp 1.0 80.2
atpF-atpA_p2 TGCAAGGAGCTCTAGGAACT CATCCCCCACTTGAACTACG 50.0 55.0 58.1 58.0 311 bp 1.0 79.9
atpF-atpA_p3 GTGCAAGGAGCTCTAGGAAC TTGTTCAATACGTTCGCGGA 55.0 45.0 58.0 58.2 257 bp 1.0 78.7
atpF-atpA_p4 TGCAAGGAGCTCTAGGAACT TTGTTCAATACGTTCGCGGA 50.0 45.0 58.1 58.2 256 bp 1.0 78.4
atpF-atpA_p5 ATTTCCGTACGATTCGTGCT CATCCCCCACTTGAACTACG 45.0 55.0 57.7 58.0 263 bp 1.0 78.1