Hotspot

APL01_gt028-APL01_gp009

Genome · 109803-110589 · 787 bp

Back to Osyris

Pi 0.0191
MarkerSeek score 64.40
Primer available yes
Label APL01_gt028-APL01_gp009

Feature Metadata

label_name
APL01_gt028-APL01_gp009
feature_type
igs
region
Genome
length_bp
787
variable_sites
15
pi
0.0191
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APL01_gt028-APL01_gp009_p1 AGCGTGTCTACCGATTTCAC TTGGACGAAGAAGGGGTACT 50.0 50.0 58.0 58.0 913-920 bp 1.0 81.2
APL01_gt028-APL01_gp009_p2 CAGCGTGTCTACCGATTTCA TTGGACGAAGAAGGGGTACT 50.0 50.0 58.0 58.0 914-921 bp 1.0 81.2
APL01_gt028-APL01_gp009_p3 AGCGTGTCTACCGATTTCAC GTTGGACGAAGAAGGGGTAC 50.0 55.0 58.0 57.9 914-921 bp 1.0 80.6
APL01_gt028-APL01_gp009_p4 CAGCGTGTCTACCGATTTCA GTTGGACGAAGAAGGGGTAC 50.0 55.0 58.0 57.9 915-922 bp 1.0 80.6
APL01_gt028-APL01_gp009_p5 TCCTAAGAGCAGCGTGTCTA TTGGACGAAGAAGGGGTACT 50.0 50.0 57.9 58.0 923-930 bp 1.0 80.2