Hotspot

APL01_gp010-APL01_gt028

Genome · 109627-109722 · 96 bp

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Pi 0.0521
MarkerSeek score 75.00
Primer available yes
Label APL01_gp010-APL01_gt028

Feature Metadata

label_name
APL01_gp010-APL01_gt028
feature_type
igs
region
Genome
length_bp
96
variable_sites
5
pi
0.0521
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APL01_gp010-APL01_gt028_p1 ACGATCGAAAAAGAGATACGAGT GTGAAATCGGTAGACACGCT 39.1 50.0 57.8 58.0 220 bp 1.0 55.9
APL01_gp010-APL01_gt028_p2 ACGATCGAAAAAGAGATACGAGT TGAAATCGGTAGACACGCTG 39.1 50.0 57.8 58.0 219 bp 1.0 55.9
APL01_gp010-APL01_gt028_p3 ACGATCGAAAAAGAGATACGAGT TGATTTCGAACAAGCCGCTA 39.1 45.0 57.8 57.9 242 bp 1.0 55.1
APL01_gp010-APL01_gt028_p4 ACGATCGAAAAAGAGATACGAGT AACAAGCCGCTATGGTGAAA 39.1 45.0 57.8 58.1 234 bp 1.0 55.1
APL01_gp010-APL01_gt028_p5 ACGATCGAAAAAGAGATACGAGT TAGACACGCTGCTCTTAGGA 39.1 50.0 57.8 57.9 210 bp 1.0 55.0