Hotspot

APL01_gp069-APL01_gp068

Genome · 12464-12514 · 51 bp

Back to Osyris

Pi 0.0196
MarkerSeek score 63.80
Primer available yes
Label APL01_gp069-APL01_gp068

Feature Metadata

label_name
APL01_gp069-APL01_gp068
feature_type
igs
region
Genome
length_bp
51
variable_sites
1
pi
0.0196
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APL01_gp069-APL01_gp068_p1 ATGCCATCGCCTACTTGAAG TGTTTGGGGCGATGAAAGAA 50.0 45.0 58.0 58.0 199-200 bp 1.0 80.0
APL01_gp069-APL01_gp068_p2 ATGCCATCGCCTACTTGAAG ACAAGGGGCTTTAGCAACTC 50.0 50.0 58.0 58.1 276-277 bp 1.0 79.3
APL01_gp069-APL01_gp068_p3 ATGCCATCGCCTACTTGAAG GGGGCTTTAGCAACTCTGAA 50.0 50.0 58.0 57.8 272-273 bp 1.0 78.4
APL01_gp069-APL01_gp068_p4 ATGCCATCGCCTACTTGAAG AAGGGGCTTTAGCAACTCTG 50.0 50.0 58.0 57.8 274-275 bp 1.0 78.3
APL01_gp069-APL01_gp068_p5 ATGCCATCGCCTACTTGAAG CAAGGGGCTTTAGCAACTCT 50.0 50.0 58.0 57.8 275-276 bp 1.0 78.3