Hotspot

APL01_gt002-APL01_gp072

Genome · 4275-5063 · 789 bp

Back to Osyris

Pi 0.00771
MarkerSeek score 64.50
Primer available yes
Label APL01_gt002-APL01_gp072

Feature Metadata

label_name
APL01_gt002-APL01_gp072
feature_type
igs
region
Genome
length_bp
789
variable_sites
6
pi
0.00771
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APL01_gt002-APL01_gp072_p1 CGAATCCCTTGCTTCATCCA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.0 58.0 947-951 bp 1.0 80.9
APL01_gt002-APL01_gp072_p2 ACGAATCCCTTGCTTCATCC AAAAAGGCGCTCAACCTACA 50.0 45.0 58.0 58.0 948-952 bp 1.0 80.9
APL01_gt002-APL01_gp072_p3 ATGGACGAATCCCTTGCTTC AAAAAGGCGCTCAACCTACA 50.0 45.0 58.0 58.0 952-956 bp 1.0 80.9
APL01_gt002-APL01_gp072_p4 GGACGAATCCCTTGCTTCAT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.0 58.0 950-954 bp 1.0 80.9
APL01_gt002-APL01_gp072_p5 CGATGGTATGGACGAATCCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 959-963 bp 1.0 80.6