Hotspot

trnR-ACG-rrn5

IRa · 135489-135749 · 261 bp

Back to Ostryopsis

Pi 0
MarkerSeek score 33.80
Primer available yes
Label trnR-ACG-rrn5

Feature Metadata

label_name
trnR-ACG-rrn5
feature_type
igs
region
IRa
length_bp
261
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5_p1 CCACGTGCTCTAATCCTCTG GGTGACGATACTGTAGGGGA 55.0 55.0 57.8 57.9 345 bp 1.0 77.8
trnR-ACG-rrn5_p2 CCACGTGCTCTAATCCTCTG AATCCATCCCGAACTTGGTG 55.0 50.0 57.8 57.9 380 bp 1.0 77.2
trnR-ACG-rrn5_p3 CCACGTGCTCTAATCCTCTG ACCAATCCATCCCGAACTTG 55.0 50.0 57.8 57.9 383 bp 1.0 77.2
trnR-ACG-rrn5_p4 CCACGTGCTCTAATCCTCTG CAATCCATCCCGAACTTGGT 55.0 50.0 57.8 57.9 381 bp 1.0 77.2
trnR-ACG-rrn5_p5 CCACGTGCTCTAATCCTCTG CACCAATCCATCCCGAACTT 55.0 50.0 57.8 57.9 384 bp 1.0 77.2