Hotspot

rpl32-trnL-TAG

SSC · 117980-118959 · 980 bp

Back to Ostryopsis

Pi 0.0092
MarkerSeek score 63.90
Primer available yes
Label rpl32-trnL-TAG

Feature Metadata

label_name
rpl32-trnL-TAG
feature_type
igs
region
SSC
length_bp
980
variable_sites
9
pi
0.0092
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-TAG_p1 ATGGAGGGGTATTGGGTAGC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.6 58.0 1104-1130 bp 1.0 76.3
rpl32-trnL-TAG_p2 GGAGGGGTATTGGGTAGCAT AGCGTGTCTACCGATTTCAC 55.0 50.0 58.6 58.0 1102-1128 bp 1.0 76.3
rpl32-trnL-TAG_p3 ATGGAGGGGTATTGGGTAGC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.6 58.0 1105-1131 bp 1.0 76.3
rpl32-trnL-TAG_p4 GGAGGGGTATTGGGTAGCAT CAGCGTGTCTACCGATTTCA 55.0 50.0 58.6 58.0 1103-1129 bp 1.0 76.3
rpl32-trnL-TAG_p5 GGAGGGGTATTGGGTAGCAT TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.6 57.9 1112-1138 bp 1.0 75.4