Hotspot

petD-rpoA

LSC · 81986-82153 · 168 bp

Back to Ostryopsis

Pi 0.0179

MarkerSeek score 62.80

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 168
variable_sites: 3
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	AGCCCTTTGGTTGGGTATTG	TGAAACATTTTCGCATAGAAGACA	50.0	33.3	58.1	57.4	306 bp	1.0	43.9
petD-rpoA_p2	CAGCCCTTTGGTTGGGTATT	TGAAACATTTTCGCATAGAAGACA	50.0	33.3	58.1	57.4	307 bp	1.0	43.9
petD-rpoA_p3	GGTTGGGTATTGGAGCAACA	TGAAACATTTTCGCATAGAAGACA	50.0	33.3	58.1	57.4	298 bp	1.0	43.7
petD-rpoA_p4	TGGTTGGGTATTGGAGCAAC	TGAAACATTTTCGCATAGAAGACA	50.0	33.3	58.1	57.4	299 bp	1.0	43.7
petD-rpoA_p5	GTCCAGTAGCGACAACTGTC	TGAAACATTTTCGCATAGAAGACA	55.0	33.3	58.3	57.4	346 bp	1.0	41.9