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psbZ-trnG-GCC

LSC · 40080-40400 · 321 bp

Back to Ostryopsis

Pi 0.0128

MarkerSeek score 62.70

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 321
variable_sites: 4
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	ATTGAACCCGCATCTTCTCC	55.0	50.0	58.0	58.0	485 bp	1.0	81.5
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCG	CCTATTACCCTGGGCGGATA	55.0	55.0	58.0	58.1	511 bp	1.0	81.3
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	ATTGAACCCGCATCTTCTCC	50.0	50.0	57.8	58.0	488 bp	1.0	80.0
psbZ-trnG-GCC_p4	TTGCTTCTCCTGATGGTTGG	CCTATTACCCTGGGCGGATA	50.0	55.0	57.8	58.1	514 bp	1.0	79.8
psbZ-trnG-GCC_p5	TCCTGATGGTTGGTCGAGTA	ATTGAACCCGCATCTTCTCC	50.0	50.0	57.8	58.0	481 bp	1.0	79.8