Hotspot

trnE-TTC-trnT-GGT

LSC · 34284-35195 · 912 bp

Back to Ostryopsis

Pi 0.0103
MarkerSeek score 68.30
Primer available yes
Label trnE-TTC-trnT-GGT

Feature Metadata

label_name
trnE-TTC-trnT-GGT
feature_type
igs
region
LSC
length_bp
912
variable_sites
9
pi
0.0103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-TTC-trnT-GGT_p1 TTCGTAGTACACCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.9 58.0 1031-1053 bp 1.0 82.2
trnE-TTC-trnT-GGT_p2 TTCGTAGTACACCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.9 58.1 1010-1032 bp 1.0 81.8
trnE-TTC-trnT-GGT_p3 GTAGTACACCTACCCCCAGG GAACCGATGACTTACGCCTT 60.0 50.0 58.2 58.0 1028-1050 bp 1.0 81.6
trnE-TTC-trnT-GGT_p4 TTCGTAGTACACCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.9 58.1 1007-1029 bp 1.0 81.6
trnE-TTC-trnT-GGT_p5 TTCGTAGTACACCTACCCCC ACTATGGTATGGCTGGGGTT 55.0 50.0 57.9 58.1 1084-1106 bp 1.0 81.3