Hotspot

psbM-trnD-GTC

LSC · 32338-33520 · 1183 bp

Back to Ostryopsis

Pi 0.00515

MarkerSeek score 63.20

Primer available yes

Label psbM-trnD-GTC

Feature Metadata

label_name: psbM-trnD-GTC
feature_type: igs
region: LSC
length_bp: 1183
variable_sites: 6
pi: 0.00515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GTC_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1276-1294 bp	1.0	59.8
psbM-trnD-GTC_p2	AGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.4	58.5	1283-1301 bp	1.0	56.7
psbM-trnD-GTC_p3	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	1277-1295 bp	1.0	55.7
psbM-trnD-GTC_p4	GGAACTAGAATGAACAGTGCAG	TTCAATTGGTCAGAGCACCG	45.5	50.0	57.1	58.5	1282-1300 bp	1.0	54.2
psbM-trnD-GTC_p5	AGAATGAACAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	45.0	52.6	55.8	57.8	1275-1293 bp	1.0	53.8