Hotspot

trnR-TCT-atpA

LSC · 11576-11838 · 263 bp

Back to Ostryopsis

Pi 0.0266

MarkerSeek score 67.80

Primer available yes

Label trnR-TCT-atpA

Feature Metadata

label_name: trnR-TCT-atpA
feature_type: igs
region: LSC
length_bp: 263
variable_sites: 7
pi: 0.0266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-TCT-atpA_p1	GGTTCAAATCCTATTGGACGC	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.1	360-366 bp	1.0	67.3
trnR-TCT-atpA_p2	GGTTCAAATCCTATTGGACGC	TTCACTGAGGAAGCAGAAGC	47.6	50.0	57.3	57.8	366-372 bp	1.0	66.6
trnR-TCT-atpA_p3	GGTTCAAATCCTATTGGACGC	AGGAAGCAGAAGCCCTTTTG	47.6	50.0	57.3	58.4	359-365 bp	1.0	65.0
trnR-TCT-atpA_p4	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	358-364 bp	1.0	65.0
trnR-TCT-atpA_p5	TGGATAGGACAGAGGTCTTCT	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	56.9	58.1	395-401 bp	1.0	64.3