Hotspot

trnG-UCC-trnR-TCT

LSC · 11193-11503 · 311 bp

Back to Ostryopsis

Pi 0.00429

MarkerSeek score 52.50

Primer available yes

Label trnG-UCC-trnR-TCT

Feature Metadata

label_name: trnG-UCC-trnR-TCT
feature_type: igs
region: LSC
length_bp: 311
variable_sites: 1
pi: 0.00429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-TCT_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	319-391 bp	1.0	67.6
trnG-UCC-trnR-TCT_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	322-394 bp	1.0	67.3
trnG-UCC-trnR-TCT_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	325-397 bp	1.0	66.9
trnG-UCC-trnR-TCT_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	328-400 bp	1.0	66.7
trnG-UCC-trnR-TCT_p5	ACCAAAGTTTCACGTTCGGT	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.3	56.9	389-461 bp	1.0	65.7