Hotspot

psbI-trnS-GCT

LSC · 9283-9434 · 152 bp

Back to Ostryopsis

Pi 0.0211

MarkerSeek score 68.90

Primer available yes

Label psbI-trnS-GCT

Feature Metadata

label_name: psbI-trnS-GCT
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 3
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCT_p1	CGTAATCCTGGACGTGAAGA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.1	58.0	240-249 bp	1.0	76.7
psbI-trnS-GCT_p2	CGTAATCCTGGACGTGAAGA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.1	58.0	241-250 bp	1.0	76.7
psbI-trnS-GCT_p3	CGTAATCCTGGACGTGAAGA	GGTTTTTCGTACCGAGGGTT	50.0	50.0	57.1	58.1	205-214 bp	1.0	76.6
psbI-trnS-GCT_p4	CGTAATCCTGGACGTGAAGA	TTTTTCGTACCGAGGGTTCG	50.0	50.0	57.1	58.2	203-212 bp	1.0	75.8
psbI-trnS-GCT_p5	CGTAATCCTGGACGTGAAGA	GGAGATGGCTGAGTGGACTA	50.0	55.0	57.1	58.2	253-262 bp	1.0	74.8