Hotspot

ycf1

SSC · 128676-134426 · 5751 bp

Pi 0.00174

MarkerSeek score 47.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5751
variable_sites: 14
pi: 0.00174
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGGTCCGTTCAAGAAAGGA	ACGTATCCACCGAGTTTATCA	50.0	42.9	58.6	56.6	280 bp	1.0	57.4
ycf1_p2	AGGGTCCGTTCAAGAAAGGA	TCTTTTACGTATCCACCGAGT	50.0	42.9	58.6	56.5	286 bp	1.0	56.8
ycf1_p3	AGGGTCCGTTCAAGAAAGGA	ACGTATCCACCGAGTTTATCAA	50.0	40.9	58.6	57.3	280 bp	1.0	55.1
ycf1_p4	AGGGTCCGTTCAAGAAAGGA	TCTTTTACGTATCCACCGAGTT	50.0	40.9	58.6	57.2	286 bp	1.0	54.5
ycf1_p5	CGATAGGGTCCGTTCAAGAA	ACGTATCCACCGAGTTTATCA	50.0	42.9	57.0	56.6	284 bp	1.0	54.3