Hotspot

rps16

LSC · 5535-6666 · 1132 bp

Back to Ostrya

Pi 0.00353
MarkerSeek score 60.00
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1132
variable_sites
6
pi
0.00353
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 GGGGGTTGTATAGAAAGGGT TGTATCGACCGAACCAATGA 50.0 45.0 56.2 56.7 1308-1332 bp 1.0 56.6
rps16_p2 GGGGGTTGTATAGAAAGGGTT TGTATCGACCGAACCAATGA 47.6 45.0 57.0 56.7 1308-1332 bp 1.0 54.9
rps16_p3 GGGGGTTGTATAGAAAGGGT ATGTATCGACCGAACCAATGA 50.0 42.9 56.2 57.2 1309-1333 bp 1.0 52.8
rps16_p4 GGGGGTTGTATAGAAAGGGT TGTATCGACCGAACCAATGAT 50.0 42.9 56.2 57.2 1308-1332 bp 1.0 52.8
rps16_p5 GGGGGTTGTATAGAAAGGGTT TGTATCGACCGAACCAATGAT 47.6 42.9 57.0 57.2 1308-1332 bp 1.0 51.0