Hotspot

clpP

LSC · 73824-75852 · 2029 bp

Pi 0.00132

MarkerSeek score 58.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2029
variable_sites: 4
pi: 0.00132
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GCATATTTGCGCGAAATCCC	CCCATTTTTACGCTTCCACA	50.0	45.0	58.6	56.6	2106-2121 bp	1.0	64.9
clpP_p2	GCATATTTGCGCGAAATCCC	ACCCATTTTTACGCTTCCAC	50.0	45.0	58.6	56.6	2107-2122 bp	1.0	64.9
clpP_p3	GCATATTTGCGCGAAATCCC	ACCCATTTTTACGCTTCCACA	50.0	42.9	58.6	58.4	2107-2122 bp	1.0	64.7
clpP_p4	GCATATTTGCGCGAAATCCC	AACCCATTTTTACGCTTCCAC	50.0	42.9	58.6	57.3	2108-2123 bp	1.0	62.8
clpP_p5	ATATTTGCGCGAAATCCCTG	CCCATTTTTACGCTTCCACA	45.0	45.0	56.9	56.6	2104-2119 bp	1.0	61.1