Hotspot

psaA-ycf3

LSC · 45374-46146 · 773 bp

Back to Ostrya

Pi 0.00259
MarkerSeek score 60.40
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
773
variable_sites
3
pi
0.00259
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TTGAGAAATGACCGGGTCTG ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.5 58.0 971-972 bp 1.0 76.1
psaA-ycf3_p2 TTTTACTTCTGGTTCCGGCG ATCAAGCCGCTGAGTATTGG 50.0 50.0 58.5 58.0 904-905 bp 1.0 76.1
psaA-ycf3_p3 TTGAGAAATGACCGGGTCTG CAGAGGCTTGGTTCGATCAA 50.0 50.0 57.5 57.9 986-987 bp 1.0 75.7
psaA-ycf3_p4 TTTTACTTCTGGTTCCGGCG CAGAGGCTTGGTTCGATCAA 50.0 50.0 58.5 57.9 919-920 bp 1.0 75.6
psaA-ycf3_p5 TTGAGAAATGACCGGGTCTG CGATCAAGCCGCTGAGTATT 50.0 50.0 57.5 58.1 973-974 bp 1.0 75.3