Hotspot

trnS-GCT-trnR-TCT

LSC · 9576-11401 · 1826 bp

Back to Ostrya

Pi 0.00219
MarkerSeek score 60.50
Primer available yes
Label trnS-GCT-trnR-TCT

Feature Metadata

label_name
trnS-GCT-trnR-TCT
feature_type
igs
region
LSC
length_bp
1826
variable_sites
6
pi
0.00219
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCT-trnR-TCT_p1 CAATCCGACGCTTTAGTCCA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1897-1913 bp 1.0 65.5
trnS-GCT-trnR-TCT_p2 AATCCGACGCTTTAGTCCAC AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1896-1912 bp 1.0 65.5
trnS-GCT-trnR-TCT_p3 CAATCCGACGCTTTAGTCCA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1903-1919 bp 1.0 64.9
trnS-GCT-trnR-TCT_p4 AATCCGACGCTTTAGTCCAC AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1902-1918 bp 1.0 64.8
trnS-GCT-trnR-TCT_p5 ACAAAAGCGGAAAGAGAGGG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.1 56.9 1957-1973 bp 1.0 64.7