Hotspot

petB

LSC · 81484-82880 · 1397 bp

Back to Ostericum

Pi 0.0081

MarkerSeek score 50.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1397
variable_sites: 29
pi: 0.0081
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CTAGATCGCGTCCAAAACCA	AACTTATGCGGTGGTTGGAG	50.0	50.0	58.0	58.2	2026-2065 bp	1.0	80.3
petB_p2	CTAGATCGCGTCCAAAACCA	TAAGCGAACCAGCCTATCCT	50.0	50.0	58.0	58.2	2054-2093 bp	1.0	80.0
petB_p3	AACAGCTCCTTTGATGGGTG	AACTTATGCGGTGGTTGGAG	50.0	50.0	58.1	58.2	1941-1980 bp	1.0	79.7
petB_p4	AACAGCTCCTTTGATGGGTG	TAAGCGAACCAGCCTATCCT	50.0	50.0	58.1	58.2	1969-2008 bp	1.0	79.4
petB_p5	CTAGATCGCGTCCAAAACCA	GTTCAAGTCACACACTCCCA	50.0	50.0	58.0	57.7	1928-1967 bp	1.0	79.1