Hotspot

trnL-UAG-ccsA

SSC · 121404-121505 · 102 bp

Back to Ostericum

Pi 0.0276

MarkerSeek score 71.50

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 8
pi: 0.0276
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	ACAATGGAAACGATCGAAAAGG	50.0	40.9	58.0	57.6	227-235 bp	1.0	66.1
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	ACAATGGAAACGATCGAAAAGG	50.0	40.9	58.0	57.6	226-234 bp	1.0	66.1
trnL-UAG-ccsA_p3	CTCTTAGGAAGCAGTGCGAG	ACAATGGAAACGATCGAAAAGG	55.0	40.9	58.1	57.6	206-214 bp	1.0	66.1
trnL-UAG-ccsA_p4	CTTAGGAAGCAGTGCGAGAG	ACAATGGAAACGATCGAAAAGG	55.0	40.9	58.1	57.6	204-212 bp	1.0	66.1
trnL-UAG-ccsA_p5	AACAAGCCGCTATGGTGAAA	ACAATGGAAACGATCGAAAAGG	45.0	40.9	58.1	57.6	241-249 bp	1.0	65.1