Hotspot

trnC-GCA-petN

LSC · 33982-34685 · 704 bp

Back to Ostericum

Pi 0.00584

MarkerSeek score 61.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 14
pi: 0.00584
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	728-760 bp	1.0	83.6
trnC-GCA-petN_p2	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	55.0	50.0	57.9	58.2	768-800 bp	1.0	83.5
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	789-821 bp	1.0	81.7
trnC-GCA-petN_p4	CGATATGGCCGAGTGGTAAG	AGAGTCCACTTCTTCCCCAT	55.0	50.0	57.9	57.6	829-861 bp	1.0	81.6
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	746-778 bp	1.0	81.4