Hotspot

trnS-GCU-trnG-UCC

LSC · 8737-9290 · 554 bp

Back to Ostericum

Pi 0.00679
MarkerSeek score 63.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
554
variable_sites
13
pi
0.00679
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA GGGGGTTACTCGAACGAATC 45.0 55.0 58.0 58.1 610-645 bp 1.0 84.8
trnS-GCU-trnG-UCC_p2 ACGGAAAGAGAGGGATTCGA GGGGGTTACTCGAACGAATC 50.0 55.0 58.2 58.1 659-694 bp 1.0 83.7
trnS-GCU-trnG-UCC_p3 ATTAGCAATCCGCCGCTTTA AGGGGGTTACTCGAACGAAT 45.0 50.0 58.0 58.4 611-646 bp 1.0 81.8
trnS-GCU-trnG-UCC_p4 GCTTTAGTCCACTCAGCCAT GGGGGTTACTCGAACGAATC 50.0 55.0 57.6 58.1 596-631 bp 1.0 81.8
trnS-GCU-trnG-UCC_p5 AGTCCACTCAGCCATCTCTC GGGGGTTACTCGAACGAATC 55.0 55.0 58.5 58.1 591-626 bp 1.0 81.0