Hotspot

rps12-rps7

IRa · 146071-146122 · 52 bp

Back to Osteomeles

Pi 0.0192

MarkerSeek score 63.60

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: IRa
length_bp: 52
variable_sites: 1
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	CTGTGGAAAGCCGTATTCGA	TTTCTTCTGCAGTACCTCGG	50.0	50.0	58.0	57.3	185 bp	1.0	74.4
rps12-rps7_p2	TCTGTGGAAAGCCGTATTCG	TTTCTTCTGCAGTACCTCGG	50.0	50.0	58.0	57.3	186 bp	1.0	74.4
rps12-rps7_p3	CTGTGGAAAGCCGTATTCGA	TTTTCTTCTGCAGTACCTCGG	50.0	47.6	58.0	58.0	186 bp	1.0	72.1
rps12-rps7_p4	TCTGTGGAAAGCCGTATTCG	TTTTCTTCTGCAGTACCTCGG	50.0	47.6	58.0	58.0	187 bp	1.0	72.1
rps12-rps7_p5	GGCTTGGAGGGAGATCTTTC	TTTCTTCTGCAGTACCTCGG	55.0	50.0	57.7	57.3	149 bp	1.0	71.9