Hotspot

rps16-trnQ-UUG

LSC · 6431-7259 · 829 bp

Back to Osteomeles

Pi 0.00121

MarkerSeek score 59.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 1
pi: 0.00121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	909-913 bp	1.0	78.2
rps16-trnQ-UUG_p2	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	919-923 bp	1.0	76.5
rps16-trnQ-UUG_p3	GCTTTCTGCCACATCGTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	886-890 bp	1.0	76.5
rps16-trnQ-UUG_p4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	920-924 bp	1.0	76.4
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GTGTCGACTAGAAATGGGGC	50.0	55.0	58.2	58.3	971-975 bp	1.0	76.0