Hotspot

trnK-UUU-rps16

LSC · 4342-5294 · 953 bp

Back to Osteomeles

Pi 0
MarkerSeek score 59.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
953
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CGGAACTTCGCCCTAATCAA 50.0 50.0 58.2 58.0 1024-1033 bp 1.0 78.8
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT CGGAACTTCGCCCTAATCAA 50.0 50.0 58.2 58.0 1023-1032 bp 1.0 78.8
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA CGGAACTTCGCCCTAATCAA 50.0 50.0 58.3 58.0 1033-1042 bp 1.0 77.9
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1079-1088 bp 1.0 77.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1078-1087 bp 1.0 77.4