Hotspot

ycf1

SSC · 125111-130717 · 5607 bp

Back to Osmanthus

Pi 0.0059

MarkerSeek score 55.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5607
variable_sites: 113
pi: 0.0059
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAGGAAGTTGCAATAGTAGCA	GAGGGGGTCAGCATTCAAAA	40.9	50.0	57.4	58.1	246-270 bp	1.0	64.0
ycf1_p2	TCAGGAAGTTGCAATAGTAGCA	AGAAATTGGGTAGGGAGGGG	40.9	55.0	57.4	58.4	260-284 bp	1.0	61.2
ycf1_p3	CAGGAAGTTGCAATAGTAGCA	GAGGGGGTCAGCATTCAAAA	42.9	50.0	56.0	58.1	245-269 bp	1.0	60.2
ycf1_p4	TTCAGGAAGTTGCAATAGTAGCA	GAGGGGGTCAGCATTCAAAA	39.1	50.0	58.1	58.1	247-271 bp	1.0	59.6
ycf1_p5	TCAGGAAGTTGCAATAGTAGCAT	GAGGGGGTCAGCATTCAAAA	39.1	50.0	57.9	58.1	246-270 bp	1.0	59.6