Hotspot

ndhF

SSC · 112229-114451 · 2223 bp

Back to Osmanthus

Pi 0.00355

MarkerSeek score 42.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 32
pi: 0.00355
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGATCCGCCGAAGAAGATC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	57.9	58.0	2492-2498 bp	1.0	72.8
ndhF_p2	ACAGCTTCCGAAATGAAGGG	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.2	58.0	2530-2536 bp	1.0	71.8
ndhF_p3	GCAGAATACCGTCACCCATT	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.0	58.0	2628-2634 bp	0.9	68.8
ndhF_p4	CAGGAACAAGAGAGATCCGC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	58.1	58.0	2503-2509 bp	0.9	68.6
ndhF_p5	GCAGAATACCGTCACCCATT	AGGTAAGATCCGGTGAATCG	50.0	50.0	58.0	56.8	2627-2633 bp	0.9	67.4