Hotspot

ycf4-cemA

LSC · 62684-63555 · 872 bp

Back to Osmanthus

Pi 0.00248

MarkerSeek score 56.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 8
pi: 0.00248
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.0	58.1	1043-1051 bp	1.0	80.3
ycf4-cemA_p2	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1042-1050 bp	1.0	80.1
ycf4-cemA_p3	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1041-1049 bp	1.0	80.1
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	968-976 bp	1.0	79.6
ycf4-cemA_p5	GAACAAAAAGCCGCCGAATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.2	58.1	997-1005 bp	1.0	78.8