Hotspot

psaA-ycf3

LSC · 43581-44318 · 738 bp

Back to Osmanthus

Pi 0.00515

MarkerSeek score 46.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 14
pi: 0.00515
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTTCTGGTTCCGGCGAAC	GGAAACAGGCTATAGCGCTT	61.1	50.0	58.4	58.0	828-856 bp	1.0	63.4
psaA-ycf3_p2	CTTCTGGTTCCGGCGAAC	ATTGGTTGAAGATCACGGGG	61.1	50.0	58.4	57.9	780-808 bp	1.0	62.9
psaA-ycf3_p3	CTTCTGGTTCCGGCGAAC	TGGTTTGATCAAGCAGCTGA	61.1	45.0	58.4	57.7	853-881 bp	1.0	61.4
psaA-ycf3_p4	CTTCTGGTTCCGGCGAAC	AGGCTATAGCGCTTACTCCT	61.1	50.0	58.4	57.7	822-850 bp	1.0	61.4
psaA-ycf3_p5	CTTCTGGTTCCGGCGAAC	TTGGAAACAGGCTATAGCGC	61.1	50.0	58.4	58.3	830-858 bp	1.0	61.0