Hotspot

rps16-trnQ(UUG)

LSC · 6448-8095 · 1648 bp

Back to Osmanthus

Pi 0.00279
MarkerSeek score 57.50
Primer available yes
Label rps16-trnQ(UUG)

Feature Metadata

label_name
rps16-trnQ(UUG)
feature_type
igs
region
LSC
length_bp
1648
variable_sites
18
pi
0.00279
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ(UUG)_p1 CGGATCTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 1734-1750 bp 1.0 79.7
rps16-trnQ(UUG)_p2 ATCTGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1731-1747 bp 1.0 79.7
rps16-trnQ(UUG)_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1720-1736 bp 1.0 79.3
rps16-trnQ(UUG)_p4 CCACAACGGATCTGTCCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1740-1756 bp 1.0 78.2
rps16-trnQ(UUG)_p5 GGATCTGTCCTTCAAGTCGC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1733-1749 bp 1.0 77.5