Hotspot

matK-rps16

LSC · 3672-5311 · 1640 bp

Back to Osmanthus

Pi 0.00341

MarkerSeek score 63.60

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1640
variable_sites: 19
pi: 0.00341
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CCCTGATCATGAGCAAGTCC	GGTGCTCAACCTACAGGAAC	55.0	55.0	58.0	58.2	1754-1856 bp	1.0	83.1
matK-rps16_p2	CCCTGATCATGAGCAAGTCC	GTGCTCAACCTACAGGAACT	55.0	50.0	58.0	57.2	1753-1855 bp	1.0	77.9
matK-rps16_p3	CCTGATCATGAGCAAGTCCA	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.3	58.2	1753-1855 bp	1.0	77.8
matK-rps16_p4	ACCCTGATCATGAGCAAGTC	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.3	58.2	1755-1857 bp	1.0	77.7
matK-rps16_p5	AACCCTGATCATGAGCAAGTC	GGTGCTCAACCTACAGGAAC	47.6	55.0	58.0	58.2	1756-1858 bp	1.0	75.4