Hotspot

ycf1

SSC · 126182-131758 · 5577 bp

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Pi 0.0309

MarkerSeek score 57.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5577
variable_sites: 172
pi: 0.0309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATTCGCGGACTATTCGTC	TCCATACGAGCCAAAAGACG	50.0	50.0	57.9	58.0	1246-1252 bp	1.0	83.7
ycf1_p2	AGTGATTCGCGGACTATTCG	TCCATACGAGCCAAAAGACG	50.0	50.0	57.9	58.0	1248-1254 bp	1.0	83.7
ycf1_p3	TGATTCGCGGACTATTCGTC	ACGGTATTCCCAACAATCCG	50.0	50.0	57.9	58.0	1292-1298 bp	1.0	83.4
ycf1_p4	AGTGATTCGCGGACTATTCG	ACGGTATTCCCAACAATCCG	50.0	50.0	57.9	58.0	1294-1300 bp	1.0	83.3
ycf1_p5	GTGATTCGCGGACTATTCGT	TCCATACGAGCCAAAAGACG	50.0	50.0	58.2	58.0	1247-1253 bp	1.0	83.3