Hotspot

ndhH-rps15

SSC · 125449-125571 · 123 bp

Back to Osbeckia

Pi 0.0813

MarkerSeek score 71.90

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 123
variable_sites: 10
pi: 0.0813
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	TGCTCTTTTCTTTTAGTTGTTACA	CGGGAATCAAAAACGCGTTA	29.2	45.0	55.2	57.4	173-174 bp	1.0	44.6
ndhH-rps15_p2	TGCTCTTTTCTTTTAGTTGTTACA	TCGGGAATCAAAAACGCGTT	29.2	45.0	55.2	59.1	174-175 bp	1.0	44.6
ndhH-rps15_p3	TGCTCTTTTCTTTTAGTTGTTACA	TTCGGGAATCAAAAACGCGT	29.2	45.0	55.2	59.1	175-176 bp	1.0	44.6
ndhH-rps15_p4	TGCTCTTTTCTTTTAGTTGTTACA	CGGGAATCAAAAACGCGTTAA	29.2	42.9	55.2	58.1	173-174 bp	1.0	44.6
ndhH-rps15_p5	TGCTCTTTTCTTTTAGTTGTTACAT	CGGGAATCAAAAACGCGTTA	28.0	45.0	55.7	57.4	173-174 bp	1.0	44.6