Hotspot

ndhG-ndhI

SSC · 121099-121550 · 452 bp

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Pi 0.0356

MarkerSeek score 74.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 452
variable_sites: 15
pi: 0.0356
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCAACGCAAAAGCCGAATAA	TCAAATCGCATTAGGTCGGT	45.0	45.0	57.7	57.3	637-667 bp	1.0	77.5
ndhG-ndhI_p2	ATTGGTCCAGGCAAATCCAT	TCAAATCGCATTAGGTCGGT	45.0	45.0	57.4	57.3	539-569 bp	1.0	76.2
ndhG-ndhI_p3	CCAACGCAAAAGCCGAATAA	TCAAATCGCATTAGGTCGGTT	45.0	42.9	57.7	58.0	637-667 bp	1.0	74.8
ndhG-ndhI_p4	ATTGGTCCAGGCAAATCCAT	TCAAATCGCATTAGGTCGGTT	45.0	42.9	57.4	58.0	539-569 bp	1.0	73.6
ndhG-ndhI_p5	CCAACGCAAAAGCCGAATAA	ATCAAATCGCATTAGGTCGGT	45.0	42.9	57.7	57.8	638-668 bp	1.0	73.4