Hotspot

ndhE-ndhG

SSC · 120341-120567 · 227 bp

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Pi 0.0283

MarkerSeek score 72.90

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 227
variable_sites: 6
pi: 0.0283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCCAGACACATAAGAGCCCT	AATTGCTGTTGCCCGTCAAT	50.0	45.0	57.7	59.0	338-353 bp	1.0	74.6
ndhE-ndhG_p2	TCCAGACACATAAGAGCCCT	TGCTTTGATAGGTGCAATTGC	50.0	42.9	57.7	57.8	353-368 bp	1.0	72.7
ndhE-ndhG_p3	TCCAGACACATAAGAGCCCT	ATTGCTGTTGCCCGTCAAT	50.0	47.4	57.7	58.3	337-352 bp	1.0	72.1
ndhE-ndhG_p4	TCCAGACACATAAGAGCCCT	GCTTTGATAGGTGCAATTGCT	50.0	42.9	57.7	57.5	352-367 bp	1.0	70.5
ndhE-ndhG_p5	TCCAGACACATAAGAGCCCT	AATTGCTGTTGCCCGTCAA	50.0	47.4	57.7	58.6	338-353 bp	1.0	70.2