Hotspot

ndhF-rpl32

SSC · 114938-115908 · 971 bp

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Pi 0.0474
MarkerSeek score 75.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
971
variable_sites
46
pi
0.0474
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGGAAGTGGAATGAGAGGT TCCAAATATTCTTACGAATATGTT 50.0 25.0 58.0 52.3 1084-1126 bp 1.0 43.3
ndhF-rpl32_p2 TGGGAAGTGGAATGAGAGGT TTCCAAATATTCTTACGAATATGT 50.0 25.0 58.0 52.3 1085-1127 bp 1.0 43.3
ndhF-rpl32_p3 TGGGAAGTGGAATGAGAGGTA TCCAAATATTCTTACGAATATGTT 47.6 25.0 57.8 52.3 1084-1126 bp 1.0 43.3
ndhF-rpl32_p4 CCACATGCGTCGAAGATTTT TCCAAATATTCTTACGAATATGTT 45.0 25.0 57.1 52.3 1152-1194 bp 1.0 43.1
ndhF-rpl32_p5 CCACATGCGTCGAAGATTTT TTCCAAATATTCTTACGAATATGT 45.0 25.0 57.1 52.3 1153-1195 bp 1.0 43.1