Hotspot

psaA-ycf3

LSC · 43385-44017 · 633 bp

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Pi 0.0182

MarkerSeek score 73.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 633
variable_sites: 11
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	765-793 bp	1.0	82.9
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TCACAAGGCGCTTCGAATAA	50.0	45.0	57.9	57.9	705-733 bp	1.0	82.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACAAGGCGCTT	50.0	45.0	57.9	58.1	712-740 bp	1.0	82.8
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	ATAGCGCTTACTCCCGGTAA	50.0	50.0	57.9	58.3	754-782 bp	1.0	80.9
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TAGCGCTTACTCCCGGTAAT	50.0	50.0	57.9	58.3	753-781 bp	1.0	80.9