Hotspot

psbZ-trnG-UCC

LSC · 37401-38011 · 611 bp

Back to Osbeckia

Pi 0.0362

MarkerSeek score 77.20

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 21
pi: 0.0362
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GACGGCTGGTCAAGTAACAA	AATCGAACCCGCATCTTCTC	50.0	50.0	58.1	58.1	746-768 bp	1.0	84.0
psbZ-trnG-UCC_p2	GACGGCTGGTCAAGTAACAA	GAATCGAACCCGCATCTTCT	50.0	50.0	58.1	58.1	747-769 bp	1.0	84.0
psbZ-trnG-UCC_p3	GACGGCTGGTCAAGTAACAA	ACTTTAACTTAACCCGGGCG	50.0	50.0	58.1	58.2	775-797 bp	1.0	82.8
psbZ-trnG-UCC_p4	GACGGCTGGTCAAGTAACAA	TAACTTAACCCGGGCGGATA	50.0	50.0	58.1	58.2	771-793 bp	1.0	82.6
psbZ-trnG-UCC_p5	ACGGCTGGTCAAGTAACAAA	AATCGAACCCGCATCTTCTC	45.0	50.0	57.7	58.1	745-767 bp	1.0	82.4