Hotspot

trnE-UUC-trnT-GGU

LSC · 31933-32706 · 774 bp

Back to Osbeckia

Pi 0.0483

MarkerSeek score 80.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 37
pi: 0.0483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	915-951 bp	1.0	82.5
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	894-930 bp	1.0	82.2
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	891-927 bp	1.0	82.0
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	877-913 bp	1.0	81.9
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	856-892 bp	1.0	81.8