Hotspot

rps16-trnQ-UUG

LSC · 5765-6840 · 1076 bp

Back to Osbeckia

Pi 0.0523

MarkerSeek score 80.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1076
variable_sites: 56
pi: 0.0523
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGCCACACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1149-1166 bp	1.0	83.4
rps16-trnQ-UUG_p2	ATCCACGAACGATCCTCTCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1172-1189 bp	1.0	83.1
rps16-trnQ-UUG_p3	GAACGATCCTCTCTGGCAAG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.1	58.1	1166-1183 bp	1.0	83.1
rps16-trnQ-UUG_p4	ACACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.7	58.1	1144-1161 bp	1.0	81.1
rps16-trnQ-UUG_p5	AAGCCACACGTTGCTTTCTA	AACAGACTGACAAATGGGGC	45.0	50.0	58.0	58.4	1211-1228 bp	1.0	80.7