Hotspot

rpl32-trnL-UAG

SSC · 105176-105716 · 541 bp

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Pi 0.0194
MarkerSeek score 56.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
541
variable_sites
36
pi
0.0194
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTCGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 595-631 bp 1.0 85.5
rpl32-trnL-UAG_p2 AGGGTTTTTCTCGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 594-630 bp 1.0 85.5
rpl32-trnL-UAG_p3 AAGGGTTTTTCTCGGCAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.2 57.8 588-624 bp 1.0 85.1
rpl32-trnL-UAG_p4 AGGGTTTTTCTCGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.2 57.8 587-623 bp 1.0 85.1
rpl32-trnL-UAG_p5 AGGGTTTTTCTCGGCAACAA GCTTGAGCACTGCTTCCTAA 45.0 50.0 58.2 58.2 608-644 bp 1.0 84.8