Hotspot

petA-psbJ

LSC · 61126-62121 · 996 bp

Back to Oryza

Pi 0.0177
MarkerSeek score 62.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
996
variable_sites
67
pi
0.0177
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAAAAGGTTCAATTGTACGA ACGGTTCATATTCTGGATTGGG 33.3 45.5 53.7 58.2 1003-1075 bp 0.954545 47.2
petA-psbJ_p2 TGAAAAGGTTCAATTGTACGA CGGTTCATATTCTGGATTGGGT 33.3 45.5 53.7 58.2 1002-1074 bp 0.954545 47.2
petA-psbJ_p3 AGAAACAGTTTGAAAAGGTTCA ACGGTTCATATTCTGGATTGGG 31.8 45.5 54.6 58.2 1013-1085 bp 0.954545 47.2
petA-psbJ_p4 AGAAACAGTTTGAAAAGGTTCA CGGTTCATATTCTGGATTGGGT 31.8 45.5 54.6 58.2 1012-1084 bp 0.954545 47.2
petA-psbJ_p5 TGAAAAGGTTCAATTGTACGAA CGGTTCATATTCTGGATTGGGT 31.8 45.5 54.5 58.2 1002-1074 bp 0.954545 47.2