Hotspot

rbcL-accD

LSC · 56087-57112 · 1026 bp

Back to Oryza

Pi 0.02
MarkerSeek score 59.70
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
1026
variable_sites
69
pi
0.02
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GGCTTCAGGGGGTATTCATG CCATTGCAATTGCCGGAAAT 55.0 45.0 58.0 58.0 2011-2103 bp 1.0 88.2
rbcL-accD_p2 GGCTTCAGGGGGTATTCATG TGCGCCTATTTCGAATCCAA 55.0 45.0 58.0 58.0 1640-1708 bp 1.0 88.2
rbcL-accD_p3 GAGCTGTATTTGCGAGGGAA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 2384-2476 bp 1.0 87.5
rbcL-accD_p4 AAGAGCTGTATTTGCGAGGG CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 2386-2478 bp 1.0 87.5
rbcL-accD_p5 GAGCTGTATTTGCGAGGGAA TGCGCCTATTTCGAATCCAA 50.0 45.0 58.0 58.0 2013-2081 bp 1.0 86.7