Hotspot

atpB-rbcL

LSC · 53850-54652 · 803 bp

Pi 0.0123

MarkerSeek score 54.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 34
pi: 0.0123
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGATCGATACGCCCTGTACT	AGTTTCTGTTTGTGGTGACA	50.0	40.0	58.0	55.3	878-898 bp	1.0	64.8
atpB-rbcL_p2	GGGAAAAGTGACATCCAGCA	AGTTTCTGTTTGTGGTGACA	50.0	40.0	58.1	55.3	912-932 bp	1.0	64.0
atpB-rbcL_p3	GGGGGAAAAGTGACATCCAG	AGTTTCTGTTTGTGGTGACA	55.0	40.0	58.2	55.3	914-934 bp	1.0	63.5
atpB-rbcL_p4	CCAGCACGGGTCCAATAATT	AGTTTCTGTTTGTGGTGACA	50.0	40.0	58.2	55.3	898-918 bp	1.0	62.9
atpB-rbcL_p5	TGATCGATACGCCCTGTACT	TGCTTTAGTTTCTGTTTGTGGTG	50.0	39.1	58.0	58.0	884-904 bp	1.0	62.0