Hotspot

trnC-GCA-rpoB

LSC · 18444-19548 · 1105 bp

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Pi 0.0135
MarkerSeek score 52.80
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1105
variable_sites
55
pi
0.0135
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1167-1242 bp 1.0 82.7
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1166-1241 bp 1.0 82.7
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG TCCCTCATTTCCATTCCGGA 50.0 50.0 58.1 58.4 1150-1225 bp 1.0 82.1
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG TTCCCTCATTTCCATTCCGG 50.0 50.0 58.1 57.6 1151-1226 bp 1.0 81.9
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG TCCGGGTATTGTGGACATTC 50.0 50.0 58.1 57.3 1168-1243 bp 1.0 79.7