Hotspot

trnD-GUC-psbM

LSC · 16622-17001 · 380 bp

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Pi 0.0237
MarkerSeek score 57.60
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
380
variable_sites
27
pi
0.0237
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTT 50.0 40.0 58.5 56.7 544-1070 bp 0.909091 65.4
trnD-GUC-psbM_p2 TTCAATTGGTCAGAGCACCG AATCATTTTGGCTGGCTGTT 50.0 40.0 58.5 56.5 546-1072 bp 0.909091 63.5
trnD-GUC-psbM_p3 TTCAATTGGTCAGAGCACCG ATCATTTTGGCTGGCTGTTT 50.0 40.0 58.5 56.5 545-1071 bp 0.909091 63.5
trnD-GUC-psbM_p4 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTTT 50.0 38.1 58.5 57.4 544-1070 bp 0.909091 63.3
trnD-GUC-psbM_p5 TTCAATTGGTCAGAGCACCG TTTGGCTGGCTGTTTTTACA 50.0 40.0 58.5 56.3 540-1066 bp 0.909091 62.6